妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2013.08.013

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (8): 1263-1268.doi: 10.11843/j.issn.0366-6964.2013.08.013

妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响

张崇志¹，刘迎春²，高峰^1*，李士栋¹，李玲瑶¹，侯先志¹

(1.内蒙古农业大学动物科学学院，呼和浩特 010018； 2.内蒙古农业大学生命科学学院，呼和浩特 010018)

收稿日期:2012-12-06 出版日期:2013-08-23 发布日期:2013-08-23
通讯作者: 高峰，副教授，E-mail:gaofeng1994@sina.com
作者简介:张崇志(1983-)，男，内蒙古海拉尔人，博士生，主要从事反刍动物营养研究，E-mail: nmzcz@yahoo.cn
基金资助:
国家自然科学基金项目（30800788；31260559）；内蒙古自然科学基金杰出青年基金项目(2013JQ02)；内蒙古自治区高等学校青年科技英才支持计划项目(NJYT-12-B09)

Effects of Maternal Undernutrition during Late Pregnancy on Growth, Development and Anti-oxidation Capability of Mongolia Ovine Fetal Liver

ZHANG Chong-zhi¹, LIU Ying-chun², GAO Feng^1*, LI Shi-dong¹, LI Ling-yao¹, HOU Xian-zhi¹

(1. College of Animal Science, Inner Mongolian Agricultural University, Hohhot 010018, China; 2. College of Life Science, Inner Mongolian Agricultural University, Hohhot 010018, China)

Received:2012-12-06 Online:2013-08-23 Published:2013-08-23

摘要/Abstract

摘要：

本试验旨在研究妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响。选择健康的蒙古绵羊42只(经同期发情受孕)，在妊娠90 d时选择6只母羊进行屠宰，其余按体重随机分配到3个组：限制组1 (0.175 MJ ME·kgw^-0.75·d^-1，n=14，RG1)、限制组2(0.33 MJ ME·kgw^-0.75·d^-1，n=12，RG2)和自由采食组(0.67 MJ ME·kgw^-0.75·d^-1，n=10，CG)，进行不同能量水平饲养。饲喂至妊娠140 d，各组再选择6只母羊进行屠宰。结果表明，RG1组胎儿重(P<0.01)、胎儿肝脏重(P<0.01)、肝脏中DNA浓度(P<0.01)、蛋白浓度(P<0.01)、总DNA含量(P<0.01)、蛋白质/DNA(P<0.01)、总抗氧化能力(P<0.01)、超氧化物歧化酶活性(P<0.05)显著低于CG组，而谷胱甘肽过氧化物酶活性(P<0.05)和丙二醛浓度(P<0.01)显著高于CG组；RG2组胎儿重(P<0.01)、肝脏重(P<0.05)、肝脏中DNA浓度(P<0.01)、蛋白浓度(P<0.01)、总DNA含量(P<0.01)、蛋白质/DNA(P<0.01)、总抗氧化能力(P<0.01)显著低于CG组，而丙二醛浓度(P<0.01)显著高于CG组。结果显示，妊娠后期营养限饲蒙古绵羊严重影响了其胎儿肝脏的生长发育和抗氧化能力，而且随着营养水平的降低，发育受阻程度加深，抗氧化防御敏感性增强。

Abstract:

This study was conducted to investigate effects of maternal undernutrition during late pregnancy on growth, development and anti-oxidation capability of Mongolia ovine fetal liver. 42 Mongolia ewes treated by estrus synchronization fecundability were selected, 6 ewes were slaughtered at the beginning of the experiment and the remaining 36 animals were allocated to three different groups: restricted group1 (RG1, 0.175 MJ ME·kgw^-0.75·d^-1, n=14), restricted group2 (RG2, 0.33 MJ ME·kgw^-0.75·d^-1, n=12) and control group (CG, ad libitum, 0.67 MJ ME·kgw^-0.75·d^-1, n=10). At 140 d of gestation, 6 representative ewes in each group were slaughtered. The results showed as follows: fetal weight(P<0.01), fetal liver weight(P<0.01), DNA concentration(P<0.01), protein concentration(P<0.01), total DNA contents(P<0.01), protein/DNA(P<0.01), T-AOC(P<0.01), SOD activity(P<0.05) in fetal liver were significantly decreased and GSH-PX activity (P<0.05), MDA concentration(P<0.01) were significantly enhanced in RG1 group compared with CG group. For RG2 group, the decreases of fetal weight(P<0.01), liver weight(P<0.05), DNA concentration(P<0.01), protein concentration(P<0.01), total DNA contents(P<0.01), protein/DNA(P<0.01), T-AOC (P<0.01) were founded and MDA concentration was significantly increased inRG2 group than that in CG group(P<0.01). In conclusion, the growth, development and anti-oxidation capability of fetal livel were affected seriously by maternal undernutrition during late pregnancy. With the decreasing of nutrition levels, the negative reactions to fetal liver became more severe and the sensitivity of anti-oxidation defense was increased.

中图分类号:

S826
S815.4

张崇志，刘迎春，高峰，李士栋，李玲瑶，侯先志. 妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响[J]. 畜牧兽医学报, 2013, 44(8): 1263-1268.

ZHANG Chong-zhi, LIU Ying-chun, GAO Feng, LI Shi-dong, LI Ling-yao, HOU Xian-zhi. Effects of Maternal Undernutrition during Late Pregnancy on Growth, Development and Anti-oxidation Capability of Mongolia Ovine Fetal Liver[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1263-1268.

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参考文献

［1］高峰. 妊娠后期限母羊对其胎儿生长发育及出生后羔羊补偿生长的影响［D］. 呼和浩特：内蒙古农业大学，2006: 54-79.

［2］GAO F, LIU Y C, HOU X Z. Effect of maternal undernutrition during late pregnancy on growth and development of ovine fetal visceral organs［J］. Asian-Aust J Anim Sci, 2009, 22(12):1633-1639.

［3］陈才勇，王恬，霍永久，等. 新生仔猪肝脏发育的肝细胞生化分析［J］. 畜牧兽医学报，2005，36(2)：153-157.

［4］PETERSIDE I E, SELAK M A, SIMMONS R A. Impaired oxidative phosphory-lation in hepatic mitochondria in growth-retarded rats［J］. Am J Physiol Endocrinol Metab, 2003, 285:E1258-E1266.

［5］JOBGEN W S, FRIED S K, FU W J, et al. Regulatory role for the arginine-nitric oxide pathway in metabolism of energy substrates［J］. J Nutr Biochem, 2006, 17:571-588.

［6］杜伟，王恬. 乳源性生物活性肽对新生IUGR仔猪肝脏抗氧化功能的影响［J］. 畜牧与兽医，2007，39(11)：12-14.

［7］GAO F, LIU Y C, ZHANG Z H, et al. Effect of maternal different energy density during prepartum on the growth performance, immunity and anti-oxidation capability of neonatal calves［J］. J Dairy Sci, 2012, 95(08):4510-4518.

［8］WANG J J, CHEN L X, LI D F, et al. Intrauterine growth restriction affects the proteomes of the small intestine, liver, and skeletal muscle in newborn pigs［J］. J Nutr, 2008, 138:60-66.

［9］GAO F, HOU X Z, LIU Y C, et al. Effect of maternal under nutrition during late pregnancy on lambs birth weight［J］. Asian-Aust J Anim Sci, 2008b, 21(3):371-375.

［10］VONNAHME K A, HESS B W, HANSEN T R, et al. Maternal undernutrition from early- to mid-gestation leads to growth retardation, cardiac ventricular hypertrophy, and increased liver weight in the fetal sheep［J］. Biol Reprod, 2003, 69:133-140.

［11］REGNAULT T R, DEVRIJER B, GALAN H L, et al. Development and mechanisms of fetal hypoxia in severe fetal growth restriction［J］. Placenta, 2007, (28):714-723.

［12］KLIONSKY B, WIGGLESWORTH J S. Production of experimental models of foetal growth retardation by inhibition of DNA or protein synthesis［J］. Br J Exp Path, 1970, 51:361-371.

［13］DANIEL V, GASCOIN-LACHAMBRE G, BOUBRED F, et al. The intensity of IUGR-Induced transcriptome deregulations is inversely correlated with the onset of organ function in a rat model［J］. PLoS ONE, 2011, 6(6):e21222.

［14］GEORGIEVA H V, GABRASHANSKA M, VENTSISLAV K, et al. Zinc supplementation against eimeria acervulina induced oxidative damage in broiler chickens［J］. Vet Med In, 2011, 10:1-7.

［15］HAMID N A A, HASRUL M A, RUZANNA R J. Effect of vitamin E on antioxidant enzymes and DNA damage in rats following eight weeks exercise［J］. Nutr J, 2011, 10:37-38.

［16］WALLACE D C. Amitochondrial paradigm of metabolic and degenerativediseases, aging, and cancer: a dawn for evolutionary medicine［J］. Annu Rev Genet, 2005, 39:359-407.

［17］肖杰, 杨帆, 崔恒敏, 等. 高钼对雏鸡肝脏和脾脏抗氧化功能的影响［J］. 畜牧兽医学报，2010，41(7)：883-890.

［18］胡锴，任常宝，颜城, 等. 高铜日粮对肉鸡肝脏抗氧化功能的影响［J］. 中国家禽，2011，33(12)：26-29.

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妊娠后期营养限饲对蒙古绵羊胎儿肝脏生长发育及抗氧化能力的影响

Effects of Maternal Undernutrition during Late Pregnancy on Growth, Development and Anti-oxidation Capability of Mongolia Ovine Fetal Liver

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